Description
Note: This product contains powder, NOT liquid. When fully dissolved in distilled water, it makes a total of 10 liters. The formula concentration is 5.36 g per liter.
Please order the Product Express post and put the product in the fridge as soon as it arrives. Do not put in the Freezer.
Expiration Standard: 3 Years from the manufacture date.
Storage Conditions: Store in the fridge at 2ยฐ-8ยฐC, avoid direct light.
DKW Medium โ Driver and Kuniyuki Walnut Medium
DKW Medium, short for Driver and Kuniyuki Walnut Medium, was initially formulated for the micropropagation of walnut using nodal explants. Today, it is widely used across various plant species due to its unique nutrient profileโcharacterized by lower nitrogen and higher sulfate concentrationsโwhich may positively influence shoot regeneration and overall plant development.
Key Benefits
Convenient and straightforward to use
Supplies essential nutrients for optimal in vitro plant growth
Formulated with reduced nitrogen and elevated sulphate levels
Supports multiple shoot induction
Enables callus-mediated shoot regeneration
How to Prepare DKW Medium (1 Litre)
Follow these steps for reliable media preparation:
In a beaker, pour 800 mL of distilled water.
Add the following:
5.36 grams of PCTโs DKW Medium powder
30 grams of sugar
1โ2 mL of PPMโข (Plant Preservative Mixture)
Top up the solution to a final volume of 1000 mL.
Adjust the pH to 5.4โ5.8 using a pH meter and appropriate reagents.
Incorporate a gelling agent:
6โ8 g/L agar or 3โ4 g/L gellan gum, and continue stirring.
Autoclave the medium at 121ยฐC and 15 psi for 20 minutes.
Your DKW medium is now ready for use!
